|
KMID : 0881720100250030269
|
|
Journal of Food Hygiene and Safety
2010 Volume.25 No. 3 p.269 ~ p.277
|
|
|
Effect of Dietary Selenium on the Colon Carcinogenesis in Male ICR Mice
|
|
Cho Min-Haeng
Kim Jun-Hyeong
Hue Jin-Joo
Kang Bong-Su
Park Hyun-Ji
Nam Sang-Yoon
Yun Young-Won
Kim Jong-Soo
Jeong Jae-Hwang
Lee Beom-Jun
|
|
|
Abstract
|
|
|
|
Selenium is an essential micronutrient for normal body function and functions as an essential constituent of selenoproteins. This study was carried out to investigate effect of selenium on the formation of colonic aberrant crypt foci (ACF) and tumor formation in a mouse model. Five-week old ICR mice were acclimated for one week and fed different selenium diet (0.02, 0.1, and 0.5 ppm) for 12 weeks. Animals received three intraperitoneal injections of azoxymethane (10 mg/kg B.W. in saline for 3 weeks), followed by 2% dextran sodium sulfate in the drinking water for a week. There were four experimental groups, including a normal control group and three different selenium levels groups. After sacrifice, the total numbers of aberrant crypt (AC) and ACF were measured in the colonic mucosa after methylene blue staining. The number of tumors was noted for tumor incidence. Liver selenium concentration was measured using ICP-AES method. Gutathione peroxidase (GPx) activity was determined using a GPx assay kit in the liver and colon. TUNEL assay and proliferating cell nuclear antigen (PCNA) staining were performed to examine the cell apoptosis and cell proliferation, respectively. Immunohistochemistry of β-catenin was also performed on the mucous membrane tissue of colon. The activity of GPx in the liver and colon was decreased in the selenium-deficient diet group while it was increased in the selenium-overloaded diet group. Apoptotic positive cells were increased in the selenium-overloaded diet group but decreased in the selenium-deficient diet group. PCNA staining area was decreased in the selenium-overloaded diet group. In addition, the β-catenin protein level in the seleniumdeficient diet group was increased but decreased in the selenium-overloaded diet group. These results indicate that dietary selenium might exert a modulating effect on colon cancer by inhibiting the development of ACF and colon tumor formation in this mouse model.
|
|
|
KEYWORD
|
|
|
Azoxymethane, AOM, dextran sodium sulfate, DSS, colon cancer, aberrant crypt foci, aberrant crypt, selenium
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|